Microbiome

Sequencing

We provide metagenomic sequencing service of bacterial consortia by 16S rRNA and fungal composition by ITS2 protocols from wide spectra of sources, including but not limited to environmental, soil, plant and food samples and technological chains to check quality or manufacturing parameters and evaluate bacteria or fungi presence depending on the task. For bacteria samples, we offer whole genome sequencing (WGS) as well.

In our facility, we possess state-of-the-art iSeq 100 sequencer (Illumina) based on complementary metal-oxide-semiconductor (CMOS) technology. This latest optimised one-channel sequencing by synthesis (SBS) technology significantly reduces sequencing costs while maintaining high data quality, allowing us to provide fast and quality sequencing service.
Our workflow starts with DNA extraction and ends with the full report generation, including bioinformatic data analysis using Bion, mothur, QIIME, DADA2 pipelines for amplicon sequencing and MetaPhlAn2, HUMAnN2 for WGS interpretation.

We are flexible partners regarding clients’ wishes and preferences and consider every order individually. We highly experienced in NGS-grade DNA extraction from different starting materials and ready to work on complicated and nontrivial tasks. Based on strong scientific ground, we are ready to participate in research projects and technology development.

Human Microbiome Sequencing

We provide metagenomic NGS of the human microbiome. Our main focus is the Human Gut Microbiome sequencing, where we offer the complete package that includes Kit for Stool collection with detailed instruction, Questionnaire, Sequencing, Data Analysis and full Report with personal recommendations about diet, lifestyle, and possible risks, based on a comprehensive comparison of your data with the healthy reference group.

Additionally, we perform DNA extraction, sequencing and data analysis of human skin, mouth or vaginal microbiome.

Use in Food Product Development

Our general aim is to develop strategies to control the dynamics of microbial consortia through the whole food processing chain and during storage, as well as development of efficient technologies for incorporation of health beneficial microbes into different foods. Diet and nutrition have been shown as the main factors shaping the gut microflora, thus foods containing probiotics and prebiotics, could support restoration of healthy gut.

Monitoring the quantitative changes in microbial consortia throughout food processing allows better comprehension and management of the microbial processes. As a result our industrial partners will be able to bring novel, streamlined and stable fermented and functional (e.g. probiotic or prebiotic) foods to the market.

Microbiome sequencing and nutrition studies

Diet and nutrition have been shown as the main factors shaping the gut microflora.

Monitoring the quantitative changes in microbial consortia throughout food processing allows better comprehension and management of the microbial processes.

Look for more information about our microbiome analysing services:

www.microbiome.ee

wwww.elsavie.com

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